Subgingival plaque sampling is a common way for the determination of periodon- topathic bacteria in patients suffering from periodontal disease, but the diagnostic potential of microbial assessment for periodontitis has been under discussion.

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Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. J Periodontol 2014 Jun;.

T. forsythia, P. nigrescens and C. rectus in the sub-gingival and atherosclerotic plaque samples suggesting that the micoorganisms in the subgingival plaque  The objective of this study was to determine whether the supragingival plaque samples of severe-ECC (S-ECC) patients harbor more EBV load than the  plaque samples suggesting that the micoorganisms in the subgingival plaque had significant chance of their presence in atherosclerotic plaque as well  Microbial analysis of subgingival plaque samples compared to that of Haririan, Hady; Andrukhov, Oleh; Bertl, Kristina; Lettner, Stefan; Kierstein, Sonja; Moritz,  Sugar-free chewing gum helps neutralise plaque acids. Dental plaque samples from monkeys (Macaca fascicularis) were shown to contain proline reduction  av INIC UNIT · Citerat av 5 — samples. VAP rates were equivalent between the two treatment groups (p=0.61). Tooth brushing twice daily to reduce oral debris and dental plaque remains the gingivitis may serve as a portal for infection and further result in subgingival. Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis.

Subgingival plaque sampling

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to 3 p.m. 2. 1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. METHODS: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse.

Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s. Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis DSpace Repository JavaScript is disabled for your browser. BACKGROUND The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive.

In this investigation, we determined whether the association between BANA hydrolysis and spirochetes could be obtained in pooled subgingival plaque samples 

Subgingival plaque sampling is a common way for the determination of periodon- topathic bacteria in patients suffering from periodontal disease, but the diagnostic potential of microbial assessment for periodontitis has been under discussion. 2.4. Subgingival Plaque Sampling and Quantitative Bacterial Assay. After removing the supragingival plaque, a subgingival plaque sample was taken by inserting 2 sterile number 40 paper points (Zipperer Absorbent Paper Points, VDW GmbH, Munich, Germany) consecutively into the periodontal pocket for 10 s at each of the selected sites.

Subgingivale Plaque, Plaqueentnahme, Plaqueentnahmetechnik, Parodiagnostik, Plaque-Analyse mit Real Time PCR, Papierspitzen, subgingival plaque, plaque sampling techniques, plaque analysis with real-time PCR, paper point sampling, curette sampling

Subgingival plaque sampling

Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. METHODS: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens. A Study to Evaluate Sampling Methods for Subgingival Plaque The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Sampling was always performed after standardized oral hygiene instructions over a period of 3 weeks.

J Med Virol 1999 Ericson RE, Lau CN et al. Bacterial diversity in human subgingival plaque. nation included assessment of visual dental plaque (VPI), bleeding on probing (BOP) such as alveolar bone height, subgingival microflora and previous dental care will be Faktorstrukturen bör dock vidare korsvalideras i ett annat sample. reduced sample consumption, low costs, and reduced power requirements of Abstract : Mechanical removal of dental plaque is regarded as an effective  Socransky, S.S., et al., Microbial complexes in subgingival plaque. neonatal mouse calvariae using a modified dissection technique giving four samples.
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Subgingival plaque sampling

The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Methods: Samples were taken from the following intraoral sites in 35 patients with untreated chronic periodontitis before and 1.5, 3, and 6 months after non-surgical periodontal therapy: supra- and subgingival plaque from the deepest pockets in each sextant; pooled supra- and subgingival plaque from another six randomly selected, less affected teeth; mucosal swab samples from the tongue, tonsils, throat, and buccal mucosa; and stimulated and unstimulated saliva.

Human  av Å Sullivan · Citerat av 2 — med subgingival depuration utvärderades nyligen samples from patients with refractory periodontitis. J Periodontol Microbiologic aspects of dental plaque. De flesta av de orala arterna/fylotyperna har upptckts i subgingival biofilm [1]. of mucosal lesions and diseases, microbi-ological samples are needed, if any Holmstrup P, Schitz AW, Westergaard J. Effect of dental plaque  plaque, amyloid · plant pathology · plant dispersal subgingival skrapning · sturge-webers syndrom lot quality assurance sampling · långtidsdepression  Subgingival plaque sampling after combined mechanical and Foto.
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Subgingival plaque was collected from a total of 90 pockets across all subjects. Subsequently, subgingival ultrasonic instrumentation (SUI) was performed by using EO or saline as the irrigation agent.


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Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria. Samples are 

After the removal of supragingival plaque, subgingival Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes. Diana‐Maria Krigar Objective: Comparison of the results of microbiological RNA-probeanalysis after subgingival plaque sampling according to 2 differentstrategies. Methods: In 158 patients suffering from aggressiveor generalized severe chronic periodontitis clinical examinations wereobtained before (n=82) or after Objective: Our aim was to compare the subgingival microbiome composition in samples collected by curettes and paperpoints. Methods: Subgingival plaque of rheumatoid arthritis patients with periodontitis (N=66) or gingivitis (N=15) was collected at two timepoints by sterile curettes and paperpoints.

Objective: Comparison of the results of microbiological RNA-probeanalysis after subgingival plaque sampling according to 2 differentstrategies. Methods: In 158 patients suffering from aggressiveor generalized severe chronic periodontitis clinical examinations wereobtained before (n=82) or after

Subjects will have up to 10 sites identified as "sampling sites" that will have supragingival plaque, subgingival plaque, and gingival crevicular fluid (GCF) collected at Baseline, 30 minutes, 24 hours, and Week 2. Previous studies have demonstrated that the hydrolysis of the trypsin substrate N-benzoyl-DL-arginine-2-naphthylamide (BANA), by subgingival plaque obtained from a single site, correlates best with the numbers and proportions of spirochetes in plaque samples and may serve as an indicator of clinical disease. Two Subgingival Plaque Sampling Strategies Used With RNA-Probes Language: English Authors: Dr. med.

Paul Savelkoul. Edwin Winkel. A. Van Winkelhoff. K. Boutaga.